Formation of spatially and geometrically controlled three-dimensional tissues in soft gels by sacrificial micromolding.

نویسندگان

  • Alec Cerchiari
  • James C Garbe
  • Michael E Todhunter
  • Noel Y Jee
  • James R Pinney
  • Mark A LaBarge
  • Tejal A Desai
  • Zev J Gartner
چکیده

Patterned three-dimensional (3D) cell culture models aim to more accurately represent the in vivo architecture of a tissue for the purposes of testing drugs, studying multicellular biology, or engineering functional tissues. However, patterning 3D multicellular structures within very soft hydrogels (<500 Pa) that mimic the physicochemical environment of many tissues remains a challenge for existing methods. To overcome this challenge, we use a Sacrificial Micromolding technique to temporarily form spatially and geometrically defined 3D cell aggregates in degradable scaffolds before transferring and culturing them in a reconstituted extracellular matrix. Herein, we demonstrate that Sacrificial Micromolding (1) promotes cyst formation and proper polarization of established epithelial cell lines, (2) allows reconstitution of heterotypic cell-cell interactions in multicomponent epithelia, and (3) can be used to control the lumenization-state of epithelial cysts as a function of tissue size. In addition, we discuss the potential of Sacrificial Micromolding as a cell-patterning tool for future studies.

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عنوان ژورنال:
  • Tissue engineering. Part C, Methods

دوره 21 6  شماره 

صفحات  -

تاریخ انتشار 2015